WebJan 22, 2008 · Background In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway® cloning technology (Invitrogen Co.) has developed a fast and reliable alternative cloning … WebGateway technology is a universal platform that enables you to shuttle your gene of interest (GOI) into as many expression and functional analysis systems as you‘d like. …
Development of the Gateway Recycling Cloning System for …
WebDec 6, 2007 · In particular, the Gateway technology developed originally by researchers at Life Technologies, Inc. ( Hartley et al., 2000 ), and now commercialized by Invitrogen, has been endorsed by a large community and compatible vectors have been created for most applications requiring the creation of recombinant DNA molecules. WebInvitrogen Corporation 1600 Faraday Avenue Carlsbad, CA 92008 T: 1 760 603 7200 F: 1 760 602 6500 E: [email protected] For country-specific contact information visit our web site at www.invitrogen.com ViraPower™ Promoterless Lentiviral Gateway® Kits Using MultiSite Gateway® Technology to clone a promoter and gene of interest into ... maritime conservatory of music halifax
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WebJan 1, 2013 · Although the Gateway ® Technology manual has previously recommended using a linearized destination vector and entry clone for more … WebAug 6, 2009 · These vectors are based on the Gateway technology (Invitrogen) whereby the cDNA, shRNA or miRNA of interest is cloned into an Entry vector and then recombined into a Destination vector that carries the chosen viral backbone and drug selection marker. WebDec 6, 2006 · Invitrogen Gateway technology exploits the integrase/ att site-specific recombination system for directional cloning of PCR products and the subsequent subcloning into destination vectors. One or three DNA segments can be cloned using Gateway or MultiSite Gateway respectively. maritime construction services netherlands